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KMID : 0378019820250070075
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New Medical Journal
1982 Volume.25 No. 7 p.75 ~ p.79
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Diagnostic Value of Alkaline-Phosphatase and Intestinal Alkaline Phosphatase Activity in Experimental Small Bowel Infarction in Rabbits
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Abstract
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The high mortality rate associated with mesenteric infarction is largely due to delay in its diagnosis and treatment. Current techniques for making the diagnosis are primarily clinical ones and often the clinical signs are atypical or absent. Unfortunately, no tests are currently available which can definitively confirm the bowel ischemia or detect the onset of its irreversible injury (Pierce & Brockenbrough, 1970).
Alkaline phosphatase activity positive granules are located in the epithelial cells of small intestine and alternations in mucosal blood supply could provoke release of intestinal alkaline phosphatase into the small bowel lumen and systemic circulation (Barnett et al., 1976).
This experiment was performed for the purpose to determine the diagnostic value of alkaline and intestinal alkaline phosphatase activity in serum and peritoneal fluid by mesenteric infarction in rabbits.
The rabbits were divided into 2 groups (total number: 20) ; sham operation group (Group) and superior mesenteric vessels ligation group (Group 1).
Alkaline phosphatase and intestinal alkaline phosphatase activity in serum and peritoneal
fluid were measured preoperatively and 2 hour, 4 hour, 6 hours after the operation. The results were as follows:
1. In serum, there were no significant increase of alkaline phosphatase and intestinal alkaline phosphatase activity in superior mesenteric vessels ligation compared to control group (P>0. 1).
2. In peritoneal fluids, the average values of alkaline phosphatase activity were significantly increased by 3. 5 times at 2 hour, 5. 2 times at 4 hour and 3. 0 times at 6 hours after superior mesenteric vessels ligation compared to control group (P>0.01).
3. In peritoneal fluids, the average values of intestinal alkaline phosphatase were increased from 20.92±3.17% to 24.26±2.65% at 2 hour after superior mesenteric vessels ligation (P<0.05), and significantly increased from 24.5r±3.56% to 45.74±4.41% at 4 hour, 21.43±2.43% to 42.04±3.96% at 6 hours after superior mesenteric vessels ligation compared to control group (P<0.01).
4. From the above mentioned results, authors conclude that the measurement of alkaline phosphatase and intestinal alkaline phosphatase aetivity in peritoneal fluids might be a useful test in early diagnosis of the mesenteric infarction.
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